After all the data was collected we ran a statistical test called Chi Squared to see if the hypothesis put foreword was correct. After running the Chi Squared Test the Group and Class hypothesis was accepted. So in conclusion the fast plants resemble Mender’s genetic experiments. The project that was conducted was the genetics of plants, this project was meant to introduce Mender’s concept Of Debris. Mendel was a geneticist that studied patterns of inheritance through true breading pea plant. One pattern that Mendel noticed was called the Debris cross, this is when there are two traits in a single cross.
Mendel crossed a true breading round and allow pea plant with a true breading wrinkled green pea plant and the results were that the Fl s’ only displayed the dominant phenotypes. Then Mendel crossed the Flu’s and he created the If’s, the results were that there was a particular ratio of 9:3:3:1 and all phenotypes were displayed. In this project we will perform a Debris cross on fast plants the phenotypes are non-purple stem, green leaf and purple stem, green leaf. We will plant and grow a Fl then cross-pollinate and plant the If’s and get a ratio.
At the end we will conduct a chi squared to see if the ratio is statistically significant to Mender’s findings. The plants that we will be studying are Wisconsin Fast Plant; this is a strain of the rapid-cycling Brassier ARPA. The reason we are using Wisconsin Fast Plant is because the life cycle is very short. During the first 1-2 days of the life cycle the seed are germinating, then after the third day the stem pushes through the soil. After 5-8 days into the life cycle the plant is above the soil and is growing the leaves and stem. After 9 days into the life cycle the plant starts growing flowers.
After 14-17 days of the life cycle the flowers being to pollinate. Then after 18-20 days of the life cycles the new seeds start to grow. And lastly from days 21-48 the plant begin to wilt and the new seeds can be harvested. The particular phenotypes that were are studying are first non-purple, green stem and the second one is purple stem, green leaf. The possible stem colors for this fast plant are: purple and green. The possible leaf colors for this fast plant are: yellow-green and green. Figure 1 The life cycle of a Fast Plant is very short after 28 days the plant will start to produce seeds.
The purpose of this lab is to use the principles of Debris crosses and predict he expected ratio Of phenotypes and then compare the expected ratio Of phenotypes to the observed ratio of phenotypes. In this lab we want to determine the dominant and recessive phenotypes. And lastly we want to see if our hypothesis is correct by performing a Chi Squared. The first week of the experiment each group was assigned to plant one quad containing 12 seeds. Each group labeled the quad they planted as Fl . Our Professor had the Parental Fast Plants on the counter so everyone could see the phenotypes.
The phenotypes of the Parental were non-purple stem, green leaf and purple stem, green leaf. Over the next week we observed our Fl Fast Plant and recoded the phenotypes. The third week is when we started pollinating the flowers. We used a bee stick to cross-pollinate the flowers. The reason we use a bee stick to pollinate is that fast plants do not self-pollinate, so bee sticks are used to successfully reproduce. Over the next week we had to pollinate the flowers two more times so the flowers could achieve maximum fertilization. In the following week we had to complete the pollination and pinch off any unopened flower buds.
Then from weeks 5-7 we monitored the seed formation. And on week 7 we took the fast plants off their watering system and let them dry for five days. Then in week 8 we collected our IF seeds and planted them in a quad. Then lastly in week 9 we observed our IF phenotypes. We found that for the group data that the Fl generation plants all had the phenotypes purple stem and green leaves. This data lead me to believe that the purple stem and green leafs are the dominant phenotypes while the green stem and yellow-green leaf is recessive. In table #1 the values number of plants in each phenotype can be found.
There were 9 plants with the Hennessey purple stem, green leaf. There was 1 plant with the phenotypes purple stem, yellow green leaf. There were 2 plants with the phenotypes non- purple stem, green leaf. There were O plants with the phenotypes non-purple stem, yellow-green leaf. In graph #1 there is a visual of this data. We found that for the group data that the IF generation plants all had the phenotypes non-purple stem and green leaf. In table #2 the values number of plants in each phenotype can be found. There were 3 plants with the phenotypes purple stem, green leaf.
There was O plants with the phenotypes purple stem, allow green leaf. There were 5 plants with the phenotypes non-purple stem, green leaf. There were 3 plants with the phenotypes non-purple stem, yellow- green leaf. In graph #2 there is a visual of this data. Table – Group Data The phenotypes from both the Fl and 5-2 crosses Phenotype Purple stem, green leaf 9 3 purple Stem, yellow green leaf Non-purple stem, green leaf 2 5 Non-purple stem, Yellow green leaf Table #2 – Class Data The phenotypes from all the IF crosses 63 Purple stem, yellow green leaf 15 54 17 Graph #1 -? Group Data The IF croup Data Graph #2 The IF Data from Class Data